An interventional fiber optic strategy, as a representative optical medical technology, is flexible, highly sensitive, minimally invasive, anti-electromagnetic, and has good biosafety. Fiber optics can approach deep cancer lesions

The quantification of microvascular blood flow velocity is pivotal in elucidating the characteristics of retinal microcirculation, and it plays a vital role in the early detection of numerous ophthalmic pathologies. However, non-i

High-resolution label-free dual-mode full-field optical coherence tomography (FFOCT) is developed for simultaneous structural and functional imaging of mouse retinas, achieving both static contrasts gained from structural refracti

Optical coherence elastography (OCE) can quantitatively obtain the viscoelasticity of tissues using rheological models and is widely applied to the clinical diagnosis of diseases. However, commonly used rheological models in OCE d

Wide-field second-harmonic generation (SHG) was used to obtain the second-harmonic signal from the entire image area for rapid imaging, despite the fact that conventional Gaussian beam illumination has low energy utilization effic

Multi-focus parallel scanning can effectively increase laser fabrication throughput. However, the conventional approach of using a spatial light modulator (SLM) to generate multi-foci and scan this fixed number of foci with galvan

Fluorescence lifetime imaging can reveal the high-resolution structure of various biophysical and chemical parameters in a microenvironment quantitatively. However, the depth of imaging is generally limited to hundreds of micromet

We present a novel noncontact ultrasound (US) and photoacoustic imaging (PAI) system, overcoming the limitations of traditional coupling media. Using a long coherent length laser, we employ a homodyne free-space Mach–Zehnder setup

Wide-field linear structured illumination microscopy (LSIM) extends resolution beyond the diffraction limit by moving unresolvable high-frequency information into the passband of the microscopy in the form of moiré fringes. Howeve

We propose a laser speckle contrast imaging method based on uniting spatiotemporal Fourier transform. First, the raw speckle images are entirely transformed to the spatiotemporal frequency domain with a three-dimensional (3D) fast

Multiphoton microscopy is the enabling tool for biomedical research, but the aberrations of biological tissues have limited its imaging performance. Adaptive optics (AO) has been developed to partially overcome aberration to resto

We present for the first time, to the best of our knowledge, a needle probe for photoacoustic viscoelasticity (PAVE) measurements at a depth of 1 cm below the sample surface. The probe uses a gradient index rod lens, encased withi

Atherosclerotic cardio-cerebral vascular disease is the most common disease that threatens human health. Many researches indicated that oxidatively modified low-density lipoprotein (ox-LDL) is a key pathogenic factor of atheroscle

Fluorescence microscopy technology uses fluorescent dyes to provide highly specific visualization of cell components, which plays an important role in understanding the subcellular structure. However, fluorescence microscopy has s

Laser speckle imaging is a common technique to monitor blood flow. The fluctuations in speckle intensity can be related to the blood flow by calculating the speckle contrast, the ratio between the standard deviation of speckle flu

In this paper, we propose and demonstrate a dual-beam delay-encoded Doppler spectral domain optical coherence tomography (OCT) system for in vivo measurement of absolute retinal blood velocity and flow with arbitrary orientation.

We proposed a nonlinear photoacoustic (PA) technique as a new imaging contrast mechanism for tissue thermal-nonlinearity characterization. When a sine-modulated Gaussian temperature field is introduced by a laser beam, in view of

Photoacoustic microscopy (PAM) has quickly developed into a noninvasive biomedical imaging technique to achieve detection, diagnosis, and monitoring. Compared with Q-switched neodymium-doped yttrium aluminum garnet or optical para

We present a deep learning approach for living cells mitosis classification based on label-free quantitative phase imaging with transport of intensity equation methods. In the approach, we applied a pretrained deep convolutional n

Photoacoustic imaging (PAI) with a handheld linear ultrasound (US) probe is widely used owing to its convenient and inherent dual modality capability. However, the limited length of the linear probe makes PAI suffer from the limit

Localized wavefront aberrations would introduce artifacts in biomedical imaging, which, however, are often neglected, as their compensations are at the cost of the field-of-view. Here, we show rarely reported local artifacts in tw

Microrobots-assisted drug delivery and surgery have been always in the spotlight and are highly anticipated to solve the challenges of cancer in situ treatment. These versatile small biomedical robots are expected to realize direc

In this study, the effects of purification, dehydration, and coagulation processes on the absorption and reduced scattering coefficients of chicken liver tissues have been investigated by using a single integrating sphere system.

Currently, it is generally known that lens-free holographic microscopy, which has no imaging lens, can realize a large field-of-view imaging with a low-cost setup. However, in order to obtain colorful images, traditional lens-free

This study investigated the feasibility of photoacoustic (PA) imaging of bone and characterization of bone features. By conducting the experiments on bovine femoral heads ex vivo, the light and ultrasonic penetration in bones was

We report on two strategies to design and implement the galvanometer-based laser-scanning mechanisms for the realization of reflectance confocal microscopy (RCM) and stimulated Raman scattering (SRS) microscopy systems. The RCM sy

The recent development of stimulated Raman scattering (SRS) microscopy allows for highly sensitive biological imaging with molecular vibrational contrast, opening up a variety of applications including label-free imaging, metaboli

Photoacoustic imaging has been developed to image the immune study at the macro scale. Macrophages play diverse roles in the acute response to infection and tissue repair. However, macrophages activities in acute inflammation at t